Enicostemma hyssopifolium, a useful medicinal herb was subjected to antimicrobial as well as phytochemical investigations. The petroleum ether, chloroform, n-Butanol, ethanol and aqueous extracts of the whole plant of Enicostemma hyssopifolium were studied for in vitro antimicrobial activity against Human pathogenic microorganisms. The antimicrobial potential of Enicostemma hyssopifolium against human pathogenic microorganisms was investigated. Percentage of sodium decreased from roots to seed, although it showed more concentration in leaf than in stem. Phosphorus showed increasing percentage from base towards apex with a slight dip in stem.