Tissue culture methods provide a novel way for the asexual multiplication of Justicia adhatoda (L) Nees. Propagation of plants through tissue culture offers a unique advantage over conventional propagation methods for mass multiplication and for conserving the germplasm. Compact callus was induced from axillary bud explants of Justicia adhatoda and the callus showed shoot organogenesis in MS+2, 4-D 0.1 mg/.l and BAP 4.0 mg/l. and root was induced in MS+ 2, 4-D 0.1mg/l.