Different varieties of Sesamum including CV.N-85 and CV. Phule-1 were screened for seed mycoflora. Twenty seven fungi were isolated from these varieties. Varietal variation was found during the investigation. Among these fungi the present of Alternaria Carthami, A. Flavus, C. globosum, Fusarium oxysporum, R. Stolonifer, A, Nigar, Rhizoctonia Leguminicola. A. Flavus, A. Carthami were maximum in all methods.