Medicinal and aromatic plants represent a consistent part of the natural biodiversity endowment of many states in India, as well as the world at large. Medicinal and aromatic plant species are widely distributed due to a variety of climatic factors and altitudinal variations coupled with varied ecological habitats. These plant species are basic ingredient of the ethno-botanical and traditional health care system. The premillinary phytochemical analysis and antimicrobial activity of different extracts (petroleum ether, chloroform, ethyl acetate, ethanol and aqueous) of leaves of Polyalthia longifolia was studied against six different bacteria by disc diffusion method. The various metabolites present in all the extracts. Among various solvent extracts studied, chloroform extract showed higher degree of inhibition followed by ethylacetate, ethanol, petroleum ether and aqueous.