Alhagi maurorum Medic (Fabaceae) is the single species of genus Alhagi found in India. Various studies have shown A. maurorum used in folk medicines. However, its potential health benefits have not been studied in details. Methanol extracts of A. maurorum from the aerial part were screened for antimicrobial. Antimicrobial activities were tested against eight microorganisms using well diffusion method. In the present study, antimicrobial activity of leaves and stem extracts of Alhagi maurorum was evaluated against four fungus Aspergillus niger, Penicillium funiculosum, Fusarium oxysporum and Trichoderma ressei and four bacterial strains Streptomyces grisveus, Staphylococcus aureus, Bacillus subtilis and Escherichia coli. It was observed that maximum zone of inhibition was in leaves (18mm) against Streptomyces grisveus while rest of the strains were found to be resistant. In case of fungus, maximum zone was observed in stem against Trichoderma ressei and Fusarium oxysporium (14mm) while other fungal colonies were found to be resistant.