Chemical fertilizers are being used in the agricultural field in order to increase production to fulfill the need of growing population. However chemical fertilizer has several harmful effects on environment as well as on human health. In the present investigation rhizobacteria strain Enterobacter cloacae strain PGLO9 has been used to observe plant (maize) growth without addition of chemical fertilizer via pot trail experiment against uninoculated control. In the experiment maize seeds variety 'JK Surabhi gold JKMH -1701 (Hybrid maize)' was treated with Enterobacter cloacae strain PGLO9 and vegetative growth parameter root length, shoot length, root biomass and shoot biomass was observed in comparison to untreated control. Significant increase in Maize (Zea mays L.) growth (root length, shoot length, root biomass and shoot biomass) was found under inoculation with strain PGLO9 as compare to uninoculated control. Therefore strain PGLO9 can prove to be an effective environment friendly microbial inoculant that can improve growth of crop and protect environment from harmful effect of chemical fertilizer.