A recent study for pteridophytic flora in the westernmost state, Gujarat of India reports the occurrence of extinct Equisetum ramosissimum Desf. subsp. debile (Roxb. Ex Vauch.) Hauke from the wild with its molecular confirmation and phylogenetic position within other species of Equisetum across the world. Phylogenetic analysis confirms that the E. ramosissimum debile is a sister taxa of the clade containing E. hyemale affine and E. x ferrissii (E. hyemale female and E. laevigatum male). Phylogenetic position of other Equisetum species obtained in the present study was also supported by the previous studies.