A new subspecies, Ophrys holoserica (Burm.f.) Greuter subsp. shoufensis subsp. novo K. Addam & M. Bou-Hamdan from the Orchidaceae family and related to the Ophrys holoserica group, is found and morphologically described as well as illustrated for the first time in Lebanon. The new subspecies share some main characteristics of Ophrys holoserica but contrasts in many taxonomic and morphological details such as the size of the flower (smaller), color of the labellum (blur yellow from up and very light brow from down), outlook (the whole flower is super fine) and habitat. The study discusses, examines, and offers taxonomic description, pertinent information, and photographs found all over 10 years of field work. It also includes observations, fresh collection, and one holotype.