Filamentous fungi are predominantly present in the phyllospheric region of the plant. The present study aims in the Biosprospection of phyllospheric microorganisms Aspergillus niger for crude Xylanase Enzyme production. The fungus isolated were then subjected to grow on different cultural conditions like Culture medium, pH, Temperature and incubation periods in order to optimize the conditions that is well suited for the maximum enzyme production. Czapek’s Dox medium with pH 6.5, temperature 35o C and Incubation period 144 hrs showed the maximum crude xylanase production.