Species of genus Trichoderma are able to improve growth of the plant and commonly used as bio fungicides typically against soil borne phytopathogenic mycoflora. The present research work was conducted with the aims to determine biomass production of Trichoderma species on different media, chitinase activity on chitin medium, seed germination test with Trichoderma on tomato seeds and their antagonistic behavior using dual culture assay against Alternaria solani on different compost including carbon rich compost, nitrogen rich compost and nutrient enriched compost were tested. The two species of Trichoderma isolated from rhizosphere of citrus, wheat and tomato of different localities of district Sargodha, Punjab, Pakistan that were identified by using ITS1 and ITS4 primers. Three strain of T. harzianum HM, HK, HC and one strain of T. asperellum TH were evaluated and all strains produced maximum mycelial biomass on Yeast Peptone Dextrose Agar (YPDA) media and Potato Dextrose Agar (PDA) while green conidia production was seen on Oatmeal Agar (OA) media. Water Agar (WA) found to be least effective for biomass production. Rapid tomato seed germination and inhibition % of Alternaria solani in dual culture assay observed maximum by T. harzianum HK as compared to all strains while carbon rich compost inoculated Trichoderma strains found to be most effective rather than nitrogen rich compost and nutrient enriched compost.