Stevia rebaudiana (Bert.) is an emerging natural, non-caloric sugar alternative and anti-diabetic plant in Pakistan. Its propagation through seed is very difficult due to bottlenecks in seed setting and seed germination. Even people did not know the exact time and method for its propagation. To contribute in filling the existing information and knowledge gaps on asexual propagation of Stevia (Stevia rebaudiana Bertoni), an experiment was conducted with objective to establish feasible propagating media combination for healthy production of Stevia cuttings. In the present study, different stem portions (apical, middle, lower), substrate media (soil, sand, silt, FYM, coirdust) and growth regulators (Control, IBA, Trichoderma varidi culture) were investigated for higher propagation productivity. The experiment was laid out in complete randomized design (CRD). All the treatments were placed under polythene sheet completely closed from all sides to maintain required humidity level i-e >90%. Recorded data on various seedling and root parameters was analyzed by applying one way ANOVA and the treatments’ means were compared for significance by Least Significance Difference (LSD) test at 0.05% P. The study revealed that apical portion of stem grown in soil + sand + silt medium and treated with IBA 500 ppm was effective in induction of better rooting (95.84%) followed by apical portion of stem grown in soil + sand + silt media and treated with Trichoderma varidi culture (87.50%). Lowest values were observed when cuttings were not treated with any growth regulator. So, after this study, combination of apical portion of stem grown in soil + sand + silt medium and treated with IBA 500 ppm could be recommended to growers to propagate Stevia with high success on commercial scale.