Anoectochilus setaceus Blume is a precious medicinal plant that contains many compounds with high pharmacological effects. In this study, the dry extract of A. setaceus collected in Vietnam was first created by fermentation from Saccharomyces cerevisiae strain and using high-performance liquid chromatography (HPLC) method to analyze the contents of some compounds in this dry extract. The results of the study determined that the contents of quercetin, isorhamnetin and ferulic acid in the dry extract of A. setaceus were 106.8 µg, 187.2 µg and 51.9 µg respectively. This has shown that the dry extract of A. setaceus that we produced has a very high pharmacological effect in supporting the treatment of human diseases.