Enumeration of trees in the Rajshahi city was carried out from July 2018 to June 2019. A total of 140 species belonging to 48 families were encountered. The major families of tree species were Caesalpiniaceae, Mimosaceae and Moraceae. Among total number of the species, 42 plants were found to be wild and 8 plants were newly acclimatized. Besides this 18 “Star Tree” have also been found. On the basis of uses, 52 trees as ornamental, 39 trees as edible fruits and 21 as timber yielding plants were found. Alangium salviifolium (L.f.) Wangerin, Haldina cordifolia (Roxb.) Ridsdale, Artocarpus lacucha Buch.-Ham, Grevillea robusta A.Cunn ex R.Br, Putranjiva roxburghii Wall. and Suregada multiflora (A.Juss.) Baill found vulnerable in the study area. However, the present work will be helpful to the researcher for the estimation of tree species diversity in the Rajshahi City.