India is the leading country in copra production and export. The process of copra making a fungal infection is the major problem and reduces the yield. Most of the copra suppliers using sulphur for fungal eradication. In the present study using herbal spray instead of sulphur for fungal eradication. Three types of fungus were isolated from infected copra. This fungus were identified as Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer under microscope using Anneline blue staining. The leaf extracts of Peltophorum pterocarpum, Melia azedarach, Ocimum sanctum leaves, Terminalia chebula seeds and juice of Allium sativum and Citrus limon juice were formulated with different ratio for poly herbal spray production. The formulated herbal spray (F1 to F6) was tested for antifungal activity against the isolated fungus like Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer. The formulation four and six were showed the maximum antifungal activity against the three fungal species. Hence these formulations were used for fungal eradication in copra production industries in future.