The presence of weeds in lowland rice plantations can cause a decrease in crop yields, so weeds must be controlled, one of the ways to control them is by using the Metsulfuron methyl herbicide. On the other hand good agricultural land must also have sufficient C-organic content to have good physical, chemical, and biological properties that support plant growth. This study aims to examine the effect of applying organic matter on the dose of methyl methulfuron herbicide application to weeds, growth and rice yields. The trial began in December 2017 until June 2018 which was carried out in the Rice Field of Cinunuk Village, Cileunyi District, Bandung Regency. Laboratory analysis was carried out at the Soil Fertility and Plant Nutrition Laboratory, Faculty of Agriculture, Padjadjaran University, Jatinangor, Sumedang Regency. Experiments were carried out using a dwi factor and carried out using a Compartmental Design Divided into a randomized complete basic design repeated three times, the main plot factor was organic material, consisting of two levels, namely: B1 (low C-organic content (1.86%) ) and B2 (high organic C content (3.5%)), and subplot factor is the dose of the methyl Metsulfuron herbicide, consisting of five levels: D0 (without herbicide), D1 (methyl methulfuron dose 0.0010 kg ai / ha), D2 (methyl methulfuron dose 0.0020 kg ai / ha), D3 (methyl metsulfuron dose 0.0030 kg ai / ha), D4 (methyl metsulfuron dose 0.0040 kg ai / ha). The response variables observed included: weed dry weight, rice plant poisoning, growth and yield of rice plants. The results showed that between the treatment of low C-organic content (1.86%) and high C-organic content (3.5%) did not show significant differences in weed dry weight of each species, weed dry weight, growth and yield of rice plants. The treatment of methyl methulfuron herbicide from a dose of 0.0010 kg a.i. /ha to a dose of 0.0040 kg a.i. /ha affects the weed dry weight, number of vegetative tillers, number of productive tillers, and yield of rice plants.