A greenhouse and field research was conducted to evaluate twenty accessions for identification of salt tolerant genotypes of sunflower (Helianthus annuus L.) as well as their characteristics. The experiment was conducted in completely randomized design with three repeats. Salinity was developed with NaCl to achieve the final levels of 3 dSm-1, 6 dSm-1 and 9 dSm-1 salinity, whereas control contained tap water. After 60 days of planting ten seedling of each accession from each treatment and replication were uprooted and data was recorded. Sunflower genotypes G-36, G-61, A-23, A-6 and A-185 performed better in both controlled and saline conditions. These genotypes showed better shoot and root growth and biomass by least concentration of Na+ and higher concentration of K+ and Cl- in leaf sap resulting in better K+:Na+