Securinega leucopyrus (Willd.) Muell, is a thorny large shrub belonging to the family Euphorbiaceae. In Ayurveda it has a lot of potential medicinal uses. It has been described by the medico-ethnobatanist as a wonderful drug for the treatment of wound healing. The aim of present study was to establish standardization parameters of male and female plants of Securinega leucopyrus since less reports are available. Pharmacognostical evaluation including examination of morphological, microscopical characters and chemomicroscopy were performed on leaves, stem and roots of both plants. Physicochemical parameters, qualitative and quantitative estimation were performed in leaves sample of both plants because only leaves have proved clinical efficacy. The study reveals the presence of rosette and prismatic crystals of calcium oxalate in leaves of both plants sample. High numbers of prismatic crystals were observed in female plants which suggest its wound healing efficacy. Phytochemical screening of the leaves sampe showed the presence of phytoconstituents like carbohydrate, saponin, protein, flavanoid, glycoside, alkaloid, hexose, reducing sugar, tannin and steroids. These studies would help to establish quality standards, purity and sample identification of S. leucopyrus.