Tylophora indica is a perennial twining herb belonging to the family Asclepiadaceae. The plant is commonly called Indian Ipecac and Kaluthai Palai in Tamil. It is used in Unani, Sidha and Ayurveda systems of medicine to treat various diseases. It is distributed from India to Sri Lanka and South East Asia. The plant has been naturalized in Mauritius and introduced in Africa. It is a valuable medicinal plant. Previous works on this plant revealed that it can be used to treat asthma, inflammation, bronchitis, diarrhoea, allergies etc. Three solvents were used to test the antimicrobial activities and to identify phytochemical compounds. Qualitative phytochemical analysis revealed the presence of alkaloids, flavonoids, phenols, saponins, steroids, glycosides, terpenoids, tannin, carbohydrates and amino acids. The antibacterial activity of different solvent extracts by the micro-dilution method revealed that T.indica leaves extract possess bioactive compounds, which inhibits the growth of microorganisms. Among the six pathogens tested the Gram positive bacteria Bacillus subtilis was most susceptible to the extracts and the rest of the pathogens showed almost similar kind of MIC values. The range of MIC to inhibit various microorganisms in order is Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogens, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhii.