Photoautotrophy is one of the main tendencies in vitro cultivation, being considered economically efficient since it reduces the chemical inputs used in the culture medium and significantly improves the quality of the seedlings that will be taken to the field. Reforestation and environmental restoration programs involving tree species can benefit from the proper use of photoautotrophic micropropagation. Myracrodruon urundeuva Allemão is a tree with great socioeconomic value due to its chemical properties, having multiple uses by local communities, which raises the need for studies to support the production of seedlings of this species. In this sense, the present research aimed to evaluate the effect of different sucrose concentrations and seal types on the growth and morphophysiological quality of M. urundeuva in vitro, to reduce production costs and improve the quality of micropropagated seedlings. M. urundeuva diaspores were inoculated into test tubes containing 10 ml of Wood Plant Medium at 50% of the salt concentration. Three sucrose concentrations (0.0, 7.5 and 15.0 g.L^-1) combined with three seal types: total with cap, total with 3M® microporous membrane and partial seal were tested. The fresh weight, height, and leaf area were evaluated and the content of the photosynthetic pigment was analyzed, as well as morphoanatomical parameters. The CO₂ concentration was higher in the microporous membrane sealed tubes. The total microporous membrane system favored increase in the growth, and when took out the sucrose the increased the carotenoids contents. The density of tector trichomes was influenced only by the sealing factor. Seedlings cultivated without sucrose in the total microporous membrane system presented higher mean for stomata density on the abaxial face. Seedlings cultivated under microporous membrane containing systems showed greater development and organization of vascular bundles. According to the results obtained in this research, the sucrose suppression in the culture medium associated with the use of microporous membranes allows to obtain plants with better morphophysiological quality, besides reducing the production costs.