Tylophora indica belongs to the family Asclepiadaceae. The plants are widely used for important medicinal purpose worldwide for its ethno-medicinal property. It is a tropical medicinal plant used in treatment of Asthma, dermatitis and rheumatism. The present study describes the phytochemical investigation of T.indica using four different solvents such as hexane, acetone, ethyl acetate and methanol. The phytochemical studies revealed the presence of alkaloids, terpenoids, steroids, flavonoids, phenols, tannins, glycosides, carbohydrates, amino acids and saponins in the plant extracts. From the GC-MS analysis in T.indica using methanol extract totally 18 compounds were identified.