Clitoria ternatea L. which is a medicinally and economically important plant belongs to Fabaceae family originated in tropical Asia. It is widely distributed throughout the humid, lowland tropics of Africa, Asia and Central America. The present investigation empirically evaluates the effects of physical and chemical mutagens on the evaluation of photosynthetic activity in terms of chlorophyll content. This article summarizes about the chlorophyll estimation and the experimental design with thirteen treatments with different concentrations of physical and chemical mutagens and the combination of both. The chlorophyll content is found to be highest in gamma irradiated plants followed by control plants. This work suggested that the physical mutagen helped Clitoria ternatea L. plants enhancing the total chlorophyll content.