Microalgae perform as a source of biologically active compounds. However, its mass production is essential to isolate the adequate quantities of the bioactive components. Growth and multiplication of three microalgae, Chlorella ellipsoidea, Chlorella vulgaris and Coelastrum sp belonging to class Chlorophyceae was studied in the laboratory, using four different culture media viz., Bold’s Basal Medium, CHU10, Walne’s and 3N BBM+Vitamin medium. The experiment was conducted for a period of 30 days under controlled conditions of temperature (25°C) and light (1 k lux). The three different microalgae shows varied growth pattern in different culture media. The alga Chlorella ellipsoidea attained maximum cell density (5.8×106 cells/ ml) in 3N BBM +Vitamin medium within 27 days. Chlorella vulgaris reached maximum cell density (26.7 x 106 cells /ml) in BBM within 18 days and the maximum growth was observed in Chu 10 medium for Coelastrum sp with a cell density of 8.72× 106 cells/ml, within 18 days. The optimized medium of each microalga can be used for their large-scale cultivation, biomass accumulation and isolation of bioactive components.