The current study was intended to screen the anti-lipolytic activity of Wrightia tinctoria seeds. Dried seeds of Wrightia tinctoria were powdered and extracted with methanol followed by screening of anti-lipolytic activity using lipid emulsion loaded Wistar rat model in vivo. Further fractionation of methanolic extract was done to prepare n-hexane, chloroform, ethyl acetate, n-butanol and remainnig aqueous fraction. All fractions were studied for in vitro pancreatic lipase inhibitory activity. The results revealed that in the rats treated with crude methanolic extract of Wrightia tinctoria, plasma triglycerides levels 3 hours after oral administration of lipid emulsion were significantly reduced compared to the untreated group. Among all tested fractions of methanolic extract of Wrightia tinctoria seeds, n-hexane fraction showed highest lipase inhibitory action i.e. 73.26±1.84 %. In conclusion to above study it has been seen that Wrightia tinctoria seeds were found to have noticable anti lipolytic action and can be effective remedy to prevent fat absoption for weight loss treatment.