Salinity stress poses a great threat to a growing population that accounts for huge agricultural loss per annum. Millets are well-known crop plants having the ability to grow well in different soil profiles under changing climatic conditions. In this view, the present study was chosen to determine the effects of NaCl stress on Panicum sumatrense (L.) CO-2 variety exposed to 25 mM, 50 mM, 75 mM, and 100 mM NaCl doses imposed on 7th DAS (days after sowing). The result showed an enhancement in total chlorophyll (Chl) constituent and a decrease in carotenoids with increase in stress doses in P. sumatrense over control one. However, increased malonaldehyde and hydrogen peroxide contents have shown a reduction insoluble protein content. The proline content elevated significantly with increased salinity level than the control one. Thus, proline accumulation results in upsurge tolerance in P. sumatrense by enhancing photosynthetic rate when subjected to NaCl treatments.