Leafy green vegetables take up an important place among the food crops as these provides promising nutrition. Alternanthera sessilis having a place with family Amaranthaceae is usually called as sessile joyweed is local to tropical and subtropical regions of the world. It is utilized as a verdant vegetable specially in Sri lanka and some Asian nations. This plant has been used in Indian traditional medicine system to cure skin diseases, night blindness, ulcer etc. Aim of the present study is Phytochemical analysis of leaf extract of Alternanthera sessilis. Phytochemicals are the bioactive compound which plays a vital role in antimicrobial activity. Phytochemical screening of methanol extract of Alternanthera sessilis shows the presence of alkaloids, terpenoid, glycosides, phenolic compounds, carbohydrates and saponin. But the chloroform extract shows the presence of alkaloids, terpenoid and phenolic compounds. This investigation will assist with planning new and increasingly strong antimicrobial medications of natural origin.