Seedling morphology of five medicinal plants of Euphorbiaceae were studied from different parts of Tripura, North-east India. Seedling characters are important and promising from the taxonomic as well as evolutionary point of view. Seedling morphology of five species of Euphorbiaceae has been investigated on the basis of their germination pattern, position of cotyledon, hypocotyl, epicotyl, and paracotyledon and eophylls development. An artificial key has been constructed for easier identification of plants in their juvenile stage. Phenogram has been prepared to investigate the correlation between the investigated taxa.