Plumbaginaceae is an angiosperm family with cosmopolitan distribution. Most of the members belong to this family are herbaceous perennial plants. Plumbago is a genus of 10-20 species of flowering plants. In Kerala three species of the genus Plumbago are found: Plumbago indica L., Plumbago zeylanica L. and Plumbago auriculata Lam. All the three species possess high medicinal values. These three species show difference in their anatomical as well as morphological features. The present study aims a comparison between the anatomical and morphological features of these three species. The study of anatomical characteristics gives an idea about the similarities and differences in their structure. Here the stem, petiole and leaves of the plants were subjected to the study. Thin sections were taken and stained. After staining microscopic images were taken and made the comparison. Various anatomical aspects were studied.