A wide range of morphological mutants were identified in Andrographis paniculata. Micro and macro-mutants play an essential role to assess the dose/concentration of mutagens. In the present study, some of the chlorophyll mutants were observed in the different dose/concentrations of gamma rays they were chlorine, albino, Xantha, and viridis. Amongst the mutagens, Morphological mutants were observed in M₂ generation with effect of dose/concentration of mutagens and such mutants were, dwarf, tall, leaves, brown seed, early, maturing, bushy type, mutants were observed in M₂ generation. Mutant and its derivatives when used in cross breeding have found to be more productive in the development of improved A. paniculata EMS provided more number of chlorophyll and morphological mutants followed by gamma rays in this study.