Fresh air is fundamental assets for living being to survive healthy but with increasing index of anthropogenic activities, the percentage of gases in the air changes. The changes in ratio of gases in air cause the several effects on living being like plants. Plants are bio-indicators for climate justice and indigenous flora has maximum capacity to absorb the polluted air. To determine the vehicular pollution impacts on the roadside plant, an array of system and protocols generated. The objective of this study was to determine the air pollution impacts on biochemical parameters (Non-Structural Carbohydrates and Proteins) and morphological changes in plants. For this purpose, nine study sites were selected along Lahore Branch Canal and two indigenous floral species (Accasia Fistula and Ficus Religiosa) selected for sampling (leaves). The time of sampling and conducting air monitoring was same to determine the effects of polluted gases at plants at that time. Concerned subject in leaves were to study the percentage of Non-Structural Carbohydrates (NSC) and Proteins in w/w%. The study reveals polluted air break the carbon bonding and proper NSC and proteins manufacturing halts due to which morphology of plants change.