Background: Adansonia digitata L. belong to family Bombacaceae is a well-known plant in the traditional medicinal system and it has been employed for the treatment of various diseases. Aim and Objectives: This study was aimed to establish the total phenolic content and antioxidant potential of A. digitata along with the HPTLC study to identify potential phytoconstituents having antioxidant activity. Materials and Methods: Preliminary phytochemical screening and total phenol content of the samples were estimated using standard procedures and antioxidant activity of root bark, stem bark, leaves and fruits of A. digitata were done by in-vitro methods (DPPH and Nitric oxide assay). HPTLC study was also carried out using Toluene: Ethyl acetate: Formic acid (5:4:1) v/v as mobile phase. Results: Results of the study revealed the presence of tannins, flavonoids, and steroids in all the parts of A.digitata. The stem bark and fruit extract produced significantly higher amounts of total phenol content compared to root bark and leaf extract. The antioxidant study demonstrated considerable effectiveness in methanolic extracts of different parts of A.digitata may be due to free radical scavenging activity. Conclusion: The results of this study show that, methanolic extract of A.digitata has antioxidant property hence can be used as an easily accessible source of natural antioxidants.