In the current study, five different leaf extracts of wild and cultivated varieties of Olea europaea were tested for oleuropein, phytochemicals, antimicrobial and antioxidant activities.Thin Layer Chromatography (TLC) showed oleuropein in methanol and ethanol extracts. Phytochemical analysis by Fourier transmission Infra-red Spectroscopy (FT-IR) showed that wild variety contains more quantity bioactive components as compared with that of cultivated variety. The antimicrobial activities determined by disc diffusion method. It was observed that ethyl acetate and methanol extracts of both varieties (wild and cultivated) of olive leaves extracts exhibited maximum activity while minimum antimicrobial activities were observed for hexane extracts. The DPPH free radical scavenging assay of ethanol extract of O. europaea showed a linear correlation with phenol contents. Ethanol extract was more effective against most of the pathogenic bacteria while aqueous and ethyl acetate extracts were found more potent against most of the fungal strains. The current study revealed that wild variety of O. europaea is as an efficient source of oleuropein and have antioxidant and antimicrobial phytochemicals.