Plants were one of the important sources of medicines since the beginning of human civilization. There is a growing demand for plant-based medicines, health products, pharmaceuticals, food supplements, cosmetics etc. Annona squamosa Linn is a multipurpose tree with edible fruits & is a source of medicinal & industrial products. Annona squamosa Linn is used as an antioxidant. It contains alkaloids, carbohydrates, fixed oils, tannins & phenolic compound. In the present study we have evaluated the antioxidant potential of seed extract of Annona squamosa. The ethanolic extract of seed has been evaluated to exhibit moderate antioxidant activity by different methods viz. total phenolic content, DPPH radical scavenging activity and H₂O₂ scavenging activity.