The present study was carried out in order to understand the ethnobotany of medicinal plants used in human health management traditionally in various diseases prevalent among the tea tribes of Dibrugarh district, Assam. Survey was conducted in tea garden areas dwelled by the local tea tribes and data was collected through personal communication, interviews, discussions and observation. A total of 20 plant species were recorded in the month of January 2020. All the recorded plant species were generally used for the ailment of common problems of daily life like cough, fever, headache, body pain, heart, dysentery etc and sometimes used for other diseases like jaundice, pneumonia, etc after diagnosis. Plant parts like leaf, bark, root, fruit, seeds etc were found to be used by different methods of preparation like direct raw consumption with water, paste, juice, curry or by mixing with other items. The investigation revealed the inherited knowledge of utilization of medicinal plants by the people from their elders, family, friends or others members of their community. The study focuses on the potentials of ethno medicinal research because there is a need for conservation of our biodiversity and documentation of these species will benefit mankind and further pharmaco-chemical investigations may be carried out for the development of drugs to fight human diseases