In the present study a simple protocol for induction of somatic embryogenesis and plantlet regeneration in Bacopa monniera (L.) Pennell is developed. The leaf explants showed extensive callus proliferation on MS medium containing 5 µM NAA in combination with 1 µM 2,4-D. The established calli derived from leaf explants were transferred on to ½ strength and full-strength MS medium lacking and containing auxins and cytokinins individually and in the ratio >1, <1 and =1. Among all these media, maximum number of somatic embryo formation was observed on MS media containing 20 µM 2,4-D in combination with 20 µM Kin (12.0 ± 0.3 per 100mg callus). Somatic embryo induction was also observed in ½ strength and full-strength MS medium lacking auxins and cytokinins. The visual observation and histological studies of the embryogenic callus showed the different developmental stages of the somatic embryogenesis, viz. globular, heart and torpedo stage.