Hibiscus rosa-sinensis (family- Malvaceae) is an ornamental perennial shrub and native of warm temperate, subtropical and tropical regions. Symptoms associated with leaf curling and little leaf of H. rosa-sinensis were observed at Sadar bazaar of Shahjahanpur, UP, India, during June 2018. Total DNA extracted from symptomatic and asymptomatic plants and further it was subjected to PCR/nested-PCR assay using phytoplasma 16S rDNA primers as well as it was also subjected to generic PCR with universal begomovirus coat-protein (CP) gene primers. Expected size of amplicons was obtained from symptomatic plants in both PCR assays but there was no any amplifications observed from asymptomatic plants. On the basis of PCR and gel electrophoresis analysis, mixed infection of begomovirus and phytoplasma was confirmed in H. rosa-sinensis from Uttar Pradesh, India.