Objective: Herbal medicines are in great demand in the developed as well as developing countries for primary healthcare because of their wide biological and medicinal activities, higher safety margins and lesser costs. The objective of present studies deals with the macroscopical and microscopical studies of stem of Diplocyclos palmatus Linn. Method: This Pharmacognostic study comprises taxonomic details, macro and microscopic characters, physicochemical details and study of phytochemical components of all successive extracts were also carried out. Results: Seeds of Diplocyclos palmatus Linn. powder showed the presence of total ash 12.14% w/w, acid insoluble ash 0.8% w/w, water soluble ash be 3.6% w/w, alcohol soluble extractive 18.85% w/w water soluble extractive 32.48% w/w and moisture content 8.9% w/w. Conclusion: The present study on Pharmacognostical investigation of stem of Diplocyclos palmatus Linn. Whole plant or stem might be useful to supplement information in regard to its identification parameters assumed significantly in the way of acceptability of herbal drugs in present scenario lacking regulatory laws to control quality of herbal drugs.