In the present investigation, asymbiotic germination of Spathoglottis plicata seeds were carried through in vitro methods for rapid propagation on KC, MS, PM and VW basal media. Amongst four basal media, PM medium gave the maximum response (86.87%) followed by MS (73.34%), VW (73.34%) and KC (46.67%) media. Minimum time was required for the initiation of germination on PM (10.30±0.27^a weeks) medium, subsequently, MS (12.20±0.32^b weeks), VW (14.73±0.35^c weeks) and KC (16.30±0.35^d weeks) media. The well-developed seedlings were acclimatized afterward transferred to pots in green house and watered regularly. Screening of secondary metabolites were conducted among the in vitro callus, shoot buds, SPSs and leaf, root, stem sample of naturally grown orchid to compare their occurrence. Naturally grown root and leaf sample of Spathoglottis plicata gave the highest precipitation in alkaloid tests followed by in vitro developed SPSs, callus and shoot buds. Anthroquinone is absent in only shoot buds and leaf while Phlobatannin is only absent in callus, shoot bud and stem. Root and SPSs possess all the tested metabolites. The occurrence of secondary metabolites in different plant parts is sporadic and uneven.