Among several metals which are used for preparing nano particles, Silver metal is u tmost important and potential metal used to prepare silver Nano-particles.The silver Nano- particles were prepared by reducing method of silver nitrate in the presence of Cymopsis tetragonoloba seed extract.For the synthesis of silver nano particle aqueous solution of (1mM) silver nitrate is prepared and mix with the methanolic extract of Cyamopsis tetragonoloba seed extract under normal condition. Conglomeration of silver Nano particles were confirmed by colour change and PH change after 24 hrs. The integrated silver Nano particles were characterized using UV-Visible spectroscopy technique. Further characterization were done with the help of XRD, FT-IR, SEM,TEM, NMR and zeta potential. The average size of the formed silver Nano-particles were found to be between 1-100nm. Electromagnetic radiation absorbance was confirmed by NMR. Antibacterial activity ofprepared silver Nano-particles done by usingCyamopsis tetragonoloba plant seeds extract and was studied against E.coli and Bacillus subtilis bacteria.