Green synthesis of Zinc Oxide Nanoparticles (ZnO NPs) has gained a greater interest as a simple, cost effective and ecofriendly. In this study, the ZnO NPs were synthesized using Vitex altissima leaves extract by the combustion method. ZnO NPs were characterized by Powder X-Ray Diffraction (PXRD), UV-Visible spectroscopy, Fourier Transform-Infrared (FTIR) spectroscopy, Scanning Electron Microscope (SEM) and Transmission Electron Microscopy (TEM) techniques. The PXRD pattern of ZnO NPs showed hexagonal wurtzite structure with average crystalline sizes of 10-50 nm. The formation of ZnO NPs was confirmed by its maximum absorbance at 373 nm in UV-Visible spectroscopy. FTIR data showed the presence of different functional groups. Morphological studies were revealed by SEM and TEM. ZnO NPs showed an effective zone of inhibition in agar well diffusion assay for pathogenic gram positive (Staphylococcus aureus) and gram negative (Escherichia coli and Pseudomonas desmolyticum) bacteria confirming efficient antibacterial activity. ZnO NPs revealed an efficient antioxidant, anticoagulant and antiplatelet activity.