Weeds are undesirable plants that are known for the decline of biodiversity and threat to native ecosystem stability. The present study deals to assess the diversity of weed species in different habitats of district Rudraprayag of Uttarakhand. The study resulted in the documentation of a total of 187 weed species belonging to 140 genera and 44 families based on field collections and observations. The maximum number of weed species were reported from wastelands followed by crop fields, roadsides and forests/ forest edges based on their distribution in different habitats. The study on the distribution of weed species is significant for effective planning and management of the ecosystem. Innovative and integrated weed management strategies must be adopted to overcome the threats imposed by the weeds.