Medicinal plants are the local heritage with global importance and have therapeutic properties because of the presence of different complex chemical substances of varying composition, which are found as secondary metabolites in one or more parts of the plants. These phytochemicals have the ability to produce a definite physiological action on the human body. Keeping this view in mind the current investigation was carried out in Syzygium caryophyllatum (L.) Alston belongs to the family Myrtaceae. It is a low altitude evergreen tree; where the stem bark is traditionally used in the treatment of diabetes mellitus. Qualitative phytochemical analysis of the bark confirms the presence of various phytochemicals like alkaloids, flavonoids, tannins, saponins, terpenoids and quinone. From the GC-MS analysis using methanol extract totally 35 compounds were identified. Among the identified compounds major bioactive compounds are 9,12,15-Octadecatrienoic acid, n-Hexadecanoic acid, β-Sitosterol, 9,12,15-octadecatrienoic acid, ethyl ester, Stigmasterol, Vitamin E, Phytol, acetate and Octadecanoic acid. These chemical compounds are considered biologically and pharmacologically important.