The present investigation deals with the antagonistic activity of several microfungi (Aspergillus fumigatus, A. ochraceus, A. terreus, A. niger, Cephalosporium sp., Fusarium oxysporum, F. solani, Helmithosporium sp., Trichoderma sp., Memonimila sp., Penicillium granulatum and Penicillium sp.) on the growth of Fusarium moniliforme. Among them Trichoderma viride showed excellent performance when compared with other soil microfungi. Penicillium sp. recorded minimum zone of inhibition by against F.moniliforme. Surprisingly, all the microfungi possess various degrees of antifungal properties against Fusarium moniliforme. The effect of culture filtrate of antagonistic fungi by using different concentrations (5, 10, 15 and 20%) treated against F.moniliforme by in-vitro method. Among the different concentration, the 20% concentration seems to be nil growth, whereas 5% concentration of potential fungal extract (filtrate) has enormous action against F.moniliforme. The antagonist potential fungal culture filtrate not only controls the F.moniliforme growth but also the fungal enzymes can suppress its growth and other parameters. The fingi of A. fumigatus fungal enzymes have an excellent performance of antagonistic activity. The effect of Trichoderma sp. fungal enzymes of amalyse, cellulose, lipase, pectinase and protease showed significant results than the A. fumigatus.