Ceropegia jaini is important medicinal plant occur in the Konkan region of the Maharashtra, India and it is rare plant. This plant was widely used in India it has the good pharmacological property. In this present study our aimed to investigate the various phytochemicals and screening of the antioxidant activity. The phytochemical preliminary screening was carried out indicate that the presence of the phytochemical like saponins, steroids, alkaloids, flavonoids and phenolics. The antioxidant capacity of the plant was studied by using the BHT as the standard for the measurement. The activity was measured by the DPPH method. This 1, 1-diphenyl-2-picryl-hydrazyl, was used as free radical scavenger.it shows good antioxidant activity. Both the leaves and flowers has good antioxidant activity. It shows the strong relationship with the antioxidant capacity of the leaves and flower. The present study shows the leaves and flower good antioxidant activity. It can be used as good source of natural free radical scavenger. This plant used as good antioxidant activity both the leaves and flower shows this activity.