The study was undertaken to screen phytochemical activity and to study antibacterial activity of aqueous and ethanolic extracts of Quercus infectoria against bacteria namely Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Phytochemical screening was done for different phytochemicals like tannins, alkaloids, phenolic compounds, glycosides, flavonoids, proteins and reducing sugar. Antibacterial activity was tested by Disc Diffusion technique. Tannins, alkaloids, phenolic compounds, glycosides and flavonoids were found to be present in both the extracts of Q. infectoria. Antibacterial activity of Q. infectoria was found to be comparable with the control antibiotics and even better for ethanolic extract against P. aeruginosa and S. aureus. The current study suggests potential of Q. infectoria as anti-bacterial agent with need of further studies on bioactive compounds in galls of Q. infectoria responsible for antibacterial activity.