The present study on the phytochemical constituents like alkaloids, flavonoids, phenols, proteins and free amino acids, saponins, sterols, terpenoids, coumarin, glycosides, quinones, and tannin were qualitatively investigated in the selected species of Enteromorpha. The biochemical composition of protein, carbohydrate, lipid, and photosynthetic pigments like chlorophyll and carotenoid were quantitatively estimated. The phytochemical, data reveals there is no significant variation among species. Coumarin, glycosides, quinine and tannins were negative in all Enteromorpha species and others are positive. The quantitative investigated results of phytochemicals reveals less significantly varies among in the all four selected species of Enteromorpha. All values are expressed in percentage; ± mean value represents standard deviation. The maximum alkaloid percentage was 2.87 in E. compressa and maximum flavanoid is 1.66% in E. Intestinalis. E. prolifera found to have maximum sugar, lipid and protein content (6.75, 2.12 and 7.97%). The phenol level (0.66%) is higher in E. Intestinalis. E. flexuosa comparatively moderate in alakloids, sugar and protein and least among flavanoids, lipids and phenols than other species. All the four species have more than 0.4 mg/g of total chlorophyll and 0.3 mg/g of carotenoids. The maximum total chlorophyll content was 0.492 mg/g in E. Intestinalis and maximum carotenoid is 0.395 mg/g in E. prolifera.