Fringed spider flower or purple cleome (Cleome rutidosperma), an important wild weed, has long been known for medicinal and nutritional activities. A close similarities of the seed of it with the mustard seed creates an aura of enthusiasm among us for isolation and analysis of the lipid. Initial qualitative analysis of seed extract showed a characteristic oil marks in filter paper. Next the moisture content of the fresh seeds of Cleome were determined and it was found to be 84.10±1.28 %. Then the fresh seeds were dried, refluxed in 3N HCL and then subjected to solvents extraction using n-Hexane. Fatty acid composition of the extracted lipid through Gas Chromatographic (GC) analysis highlighted that the n-hexane fraction contains 41 peaks. 20 fatty acids namely, Linolelaidic acid methyl ester, Cis9-oleic acid methyl ester etc. were identified among them. Relatively abundance of low amount of saturated fatty acid and predominance of unsaturated fatty acids may also make the Cleome seed oil useful for different anthropogenic uses.