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VOL. 5, ISSUE 6 (2020)
Effect on PGPR (Plant Growth Promoting Rhizobacteria) in phytoremediation of heavy metal contaminated soil using Vigna radiata.L
Authors
Santhakumari R, Lalitha S
Abstract
Plant growth promoting rhizobacteria (PGPR) may help reducing the toxicity of heavy metals to plants in polluted soils. In this work the effects of inoculating metal resistant and plant growth promoting bacterial strains on the growth Vigna radiata L. grown in cadmium contaminated soils were assessed. The PGPR strains Bacillus subtilis and Pseudomonas fluorescens isolated from rhizosphere soil was used for bioremediation. These isolates were characterized for their ability of biochemical tests. Bacillus subtilis and Pseudomonas fluorescens were inoculated in Vigna radiata L. plants by pots inoculation method and analysed for the growth promotion efficacy in heavy metal polluted soil. The plant inoculated with isolates showed Plant growth parameters and biomass, as compared to non-inoculated control. Further analyses of leaves were carried out for the photosynthetic pigment analysis, phytochemical analysis. Heavy metal analysis of plants and soil in Vigna radiata L. Bacterial strains diversity decreased with increasing levels of metal contamination in the soil, but in rhizosphere soil of plants inoculated with the PGPR strains, a higher bacterial diversity was kept throughout the experimental period. Inoculation of Vigna radiata L. particularly Bacillus subtilis with Pseudomonas fluorescens, appears to be an effective way enhancing the short term rhizoremediation potential of the plant in metal contaminated, lowering losses in plant biomass and degreasing above ground tissue contamination. The study concluded that heavy metal mobilizing PGPR could be used as effective inoculants for improving the phytoremediation in heavy metal contaminated soil as well as reclamation of heavy metal polluted soil.
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Pages:555-563
How to cite this article:
Santhakumari R, Lalitha S "Effect on PGPR (Plant Growth Promoting Rhizobacteria) in phytoremediation of heavy metal contaminated soil using Vigna radiata.L". International Journal of Botany Studies, Vol 5, Issue 6, 2020, Pages 555-563
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