Hemidesmus indicus (L.) R. Br. ex Schult. Belongs to family Apocynaceae. It is a perennial, slender, twinning undershrub with woody fragrant roots and majorly contains phyto-constitutes such as hexatriacontane, lupeol, α-amyrin, β-amyrin and sitosterol. The roots of the drug used as coolant and a blood-purifier in traditional systems of medicines having properties like emetic, diuretic, anti-inflammatory, etc. Due to its over exploitation, limitations for natural regeneration the said drug is in the line of scarcity in nature. For fulfilling the demand of industry it is essential to take study on propagation, cultivation and conservation. The morphological, anatomical and phytochemical characteristics are essential for determining the taxa at taxonomic level and drug authentication. In the present study an attempt has been made to identify alkaloids, protein, starch, glucoside and tannin by using histochemical tests of different reagents in root, stem and leaf. All the histochemicals tests indicated presence of above mention constituents in the studied plant parts except fats and glucoside. Phytochemical study was carried out for detection of active constituents like alkaloids, flavonoids, glycosides, anthroquinones, amino acids, protein, saponins and tannins. Among all five solvents viz. ethanol, methanol, n-hexane, dichloromethane and aqueous extract, ethanol showed positive tests in all plant parts. The present result can be used for the standardization of drug at the time of utilization by the pharmaceutical industry.