Antibiotic treatment is a preferred choice to cure the microbial diseases. But long time consumption of antibiotics has been caused to formation of multi drug resistant microorganisms. The present study was aimed to determine the anti-microbial activity of methanol leaf extracts of five medicinal plants of Sri Lanka against the clinically isolated and confirmed human pathogens. A disc diffusion assay was conducted to determine minimum inhibitory concentration (MIC) and the synergetic antimicrobial activity. The MIC value for methanol extract of B. ceylanica, P. pinnata and C. cauliflora was within a range of 0.025g/ ml – 0.2g/ml. Among the plants tested, B. ceylanica was the most promising plant to inhibit the growth of MRSA (17.5±0.65 mm) and S. aureus (18.5±0.29 mm) and this was higher than the value of positive control. L. zeylanica had comparatively less antimicrobial potential than the B. ceylanica but, it showed variable antimicrobial activities against all selected human pathogens. There were tannins, phenols, flavonoids and alkaloids in various qualitative representations in all selected plants. The study showed a potent and diverse antimicrobial activities of the plants against selected human pathogens.