In Vidarbha region of Maharashtra State, soybean crop is harvested in October-November. The seeds of soybean crops are stored for 7-8 months prior to sowing. Through sun drying after harvest, followed by storage, has been found to reduce the problem of loss of viability. Here the greater part of storage period is covered by the dry and cool winter months. Even keeping the seeds under ambient conditions in ordinary gunny bags would result in significant loss of viability. Electrical conductivity test is one of the most significant tests to estimate the leakage of electrolytes from the seed coat with respect to age, storage life, and environmental factors such as temperature, humidity and mother plant nutrition. Also, during storage high seed moisture level increases seed mycoflora, which play an important role in deterioration of soybean seed quality and viability during storage. In the present work study of effect of storage materials such as Polyethylene bag, Cloth bag and Jute bag on electrical conductivity and moisture content during 540 days of storage of four different verieties such as JS-335, AMS-99-33, TAMS-38 and TAMS-98-21. Electrical conductivity was found higher in the variety TAMS-98-21 (2.288 mmoh/cm) as compared to TAMS-38, AMS-99-33 and JS-335 as 2.217 mmoh/cm, 2.126 mmoh/cm and 1.945 mmoh/cm respectively after storage of 540 days in Jute bag. The seeds stored in Polyethylene bag showed minimum variation in electrical conductivity as compared to Cloth and Jute bag. The moisture content of seeds increased or decreased according to the atmospheric relative humidity and temperature. It was found that when seeds were stored in Cloth and Jute bags, it showed higher moisture content than those stored in Polyethylene bags. The least amount of moisture increase (9.1%) was found in seeds stored in Polyethylene bag at the end of 540 days of storage. In the present investigation, the corresponding moisture content values of soybean varieties JS-335, AMS-99-33, TAMS-38 and TAMS-98-21 when stored in Polyethylene, Cloth and Jute bags were different. Variety JS-335 seeds recorded lower moisture content (9.1%) as compared to AMS-99-33, TAMS-38 and TAMS-98-21.